Anticancer Profiling of Gambogic Acid as a Target Specific RANKL Inhibitor in Osteosarcoma Cell Line

نویسندگان

چکیده

Osteosarcoma is a common cancer type among the youth population and usually develops in growing bones. Approximately twenty percent of osteosarcoma cases show tendency to metastases patients with have low survival rate after treatment. RANK/RANKL/OPG key regulator triad bone remodeling play critical roles tumourigenesis osteosarcoma. Anti-proliferative activity gambogic acid was determined Saos-2 cell line by XTT assay. To understand anticancer activities acid, molecular docking calculations were also performed RANK-RANKL complex. The expression levels RANKL OPG measured at gene protein level RT-PCR ELISA assays. Its potential anti-invasive property in-vitro against cells evaluated using wound healing Experimental assays indicated that suppressed proliferation, migration, decreased ratio RANKL/OPG cells. Gambogic binds complex Kd value 549.38 nM estimated free energy binding -8.54 kcal/mol. found be significant drug template for target specific

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ژورنال

عنوان ژورنال: Erzincan University Journal of Science and Technology

سال: 2021

ISSN: ['1307-9085', '2149-4584']

DOI: https://doi.org/10.18185/erzifbed.928668